Fluorescence Microscopy of Cells That Are Labeled with a Green

Multiple Choice

Fluorescence microscopy of cells that are labeled with a green fluorescent antibody against microtubules (a cytoskeletal component) differs from cells expressing green fluorescent protein (GFP) tagged to beta tubulin (a component of microtubules) in all the following ways EXCEPT:

A) the antibody labeled cells need to be permeabilized before visualization,while the GFP cells do not.
B) the microtubules could change their localization over time in the GFP cells,but not in the antibody labeled cells.
C) the GFP cells do not need to be treated with fixatives,but the antibody labeled cells do.
D) the antibody labeled cells will identify the microtubule structures,while the GFP will be observed in other places in the cell because it is not as specific as an antibody.