"3C" or Chromosome Conformation Capture Methods,used to Determine the Three-Dimensional

Multiple Choice

"3C" or chromosome conformation capture methods,used to determine the three-dimensional spatial organization of chromatin within nuclei of interphase cells,rely on a series of steps where the end result is the sequence analysis of purified DNA fragments.Which one of the following presents the correct order of steps you as an investigator need to follow in a 3C method strategy?

A) shear DNA to 200-600 bp;cross-link proteins and DNA with formaldehyde
B) ligate linkers marked with biotin onto DNA fragments;dilute and ligate the fragments
C) cross-link streptavidin to DNA;purify and shear biotin-labeled fragments
D) none of the above