Essay
Pull-down assays can be used to detect when small G proteins like Rac1 have been activated by various growth factors.If you were given two lysates,one having been treated with platelet-derived growth factor (PDGF)and the other treated with PDGF that had been heat-inactivated,briefly describe the pull-down assay and what you would expect to see following the assay.
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Active Rac1 (i.e. ,in the GTP-bound form...View Answer
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