Place the Five Steps in Building a Phylogenetic Tree in the Proper

Short Answer

Place the five steps in building a phylogenetic tree in the proper order,from first construction step to last.
The nucleotide or amino acid sequence must be aligned.
Application of a selected method,using computer analysis.
The alignment must be examined for a phylogenetic signal.
Manual examination of the tree.
Choose which tree-building method to use (common ones are phenetic or cladistic).