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Which PCR Step Causes the Denaturation of Double-Stranded DNA

Question 5

Multiple Choice

Which PCR step causes the denaturation of double-stranded DNA?


A) Add DNA polymerase and nucleotides at 72°C.
B) Cool DNA to between 50°C and 65°C.
C) Add primers.
D) Heat target DNA to 94°C.
E) Repeat the cycle of heating and cooling.

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