There Are "Promiscuous Plasmids," Plasmids That Can Replicate in Several

Multiple Choice

There are "promiscuous plasmids," plasmids that can replicate in several different species of bacteria.Frequently these plasmids have transposons carrying antibiotic resistance.In a hospital that has an outbreak of a variety of bacteria carrying resistance to streptomycin, gentamicin, and penicillin how what is the best way to determine that this resistance was due to a shared plasmid?

A) Demonstrate that all the resistant bacteria have a plasmid and that these plasmids have an identical (or nearly identical) sequence..
B) Isolate plasmids from all resistant bacterial species and demonstrate that sensitive cells from these same species become resistant when this plasmid is transformed..Confirm that these are the same plasmids by sequencing.
C) Sequence the genomes of the resistant sspecies and demonstrate mutations in the same homologous genes..
D) Isolate any plasmids from one resitant species and transform a sensitive strain of the same bacterium to see if resistance occured in the transformants.