When DNA Probes Are Used to Identify Bacterial DNA Similarities

Multiple Choice

When DNA probes are used to identify bacterial DNA similarities by hybridization,the probe DNA is heated and the template DNA is treated to separate the two strands.Why would the probe DNA be heated?

A) This is the only way to properly label the probe DNA.
B) The probe may contain portions that are double-stranded.Heating it up breaks any possible hydrogen bonds that may have formed allowing the single-stranded sequences to hybridize with their complementary targets.
C) Heating it up activates the tag on the probe DNA before it hybridizes to the bacterial template DNA.
D) DNA hybridization can only take place at high temperatures,so all the DNA must be heated up prior to hybridization.