In a FISH Experiment,what Would Happen If Unbound Probe Was

Multiple Choice

In a FISH experiment,what would happen if unbound probe was not washed off?

A) Nothing-it's not necessary to wash off the unbound probe.
B) You would get false-positive results in different areas where the probe hadn't actually bound,but it was still sitting there and lighting up.
C) Your FISH would be floating at the top of the tank due to the toxicity of the probe building up within them.
D) Nothing-the target nucleotide sequences are labeled,not the probe.Therefore,excess unbound probe wouldn't matter for the experiment.