In the Yeast Two-Hybrid Analysis Technique, in Vivo Protein-Protein Interactions

Multiple Choice

In the yeast two-hybrid analysis technique, in vivo protein-protein interactions are explored. The "bait" and "prey" proteins are translationally fused to the cleaved activation and DNA-binding domains of the GAL4 transcription factor. What is the target gene of GAL4?

A) lacZ
B) gadA/B
C) gal1
D) all of the above
E) none of the above