A Major Advantage of Real-Time PCR Is That It Can

Multiple Choice

A major advantage of real-time PCR is that it can be used to quantify the original amount of RNA in a sample. What additional step must be accomplished in this particular application?

A) careful design of specific forward and reverse primers
B) RNA must first be converted to cDNA
C) RNA must be denatured with formaldehyde to avoid secondary structures
D) separation of mRNA from other RNA species
E) none of the above