How Were the Sequences That Compose the Replication Origins in Yeast

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The Answer is Yeast cells were grown in heavy isotope media to produce denser DNA and then shifted to media with normal,light isotopes.After a few minutes,DNA from these cells was extracted and cut into small pieces.The lighter DNA fragments (which should contain the origins of replication)were collected,fluorescently labeled,and used to hybridize a microarray of yeast sequences.Determination of the sequences to which this light,fluorescently labeled DNA hybridized led to the identification of a number of yeast replication origins.

How Were the Sequences That Compose the Replication Origins in Yeast

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How Were the Sequences That Compose the Replication Origins in Yeast

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