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If You Are Setting Up a PCR Reaction,how Much Extension

Question 23

Multiple Choice

If you are setting up a PCR reaction,how much extension time per cycle should you allocate for the Taq DNA polymerase to amplify a 5-kb DNA fragment from a 10-kb template?


A) 1 minute
B) 2.5 minutes
C) 5 minutes
D) 10 minutes
E) 20 minutes

Correct Answer:

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