If RNA Is to Be Used in a PCR Amplification

Multiple Choice

If RNA is to be used in a PCR amplification procedure,what is the initial step that must be performed?

A) The RNA must be denatured to form single strands for annealing to primers.
B) RNA should never be used in a PCR reaction because thermostable enzymes cannot synthesize strands to anneal to it.
C) A reverse transcription procedure must be performed to form cDNA.
D) RNA must first be treated with RNases to remove interfering substances from the target.