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A Peptide Was Treated with Protease Enzymes and Cyanogen Bromide

Question 15

Essay

A peptide was treated with protease enzymes and cyanogen bromide followed by complete hydrolysis of the resulting products. Based upon the following data, determine the sequence of the original peptide.
Chymotrypsin gave two peptides containing the following amino acids:
Leu, Pro
Asp, Gln, Arg, Met, Tyr, Val
Cyanogen bromide gave two peptides containing the following amino acids:
Asp, Met
Gln, Leu, Arg, Tyr, Pro, Val
Trypsin gave two peptides containing the following amino acids:
Gln, Leu, Tyr, Pro
Asp, Arg, Met, Val

Correct Answer:

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Chymotrypsin treatment shows the C-term ...

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