Essay
You are interested in receptor protein-tyrosine kinases and you are purifying a novel phosphotyrosine-binding protein (NPBP-1).In order to characterize this protein you need to separate it from three other proteins (X, Y, and Z)that are still present in your partially purified material.The proteins in your preparation have the following properties:
a.What type of separation technique can be used to separate protein NPBP-1 from protein X?
b.What type of separation technique can be used to separate protein NPBP-1 from protein Y?
c.What type of separation technique can be used to separate protein NPBP-1 from protein Z?
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