In Eukaryotes,which of the Following Would Decrease the Rate of Transcription

Multiple Choice

In eukaryotes,which of the following would decrease the rate of transcription?

A) mutation in the nucleotide sequences at −10 and −35
B) mutation in the nucleotide sequences at −10 and +5
C) mutation in the nucleotide sequences at −70 and −90
D) mutation at the $\sigma$ binding site
E) all of the above