Essay
You found a mouse gene sequence containing an open reading frame that should produce a 43 kDa protein.You express this protein in cultured mouse cells by transforming the cells with a plasmid bearing your gene of interest.When you isolate proteins from these cells and probe for your protein of interest,you find that the detected protein is 49 kDa.Why might your protein be larger than expected when analyzed by immunoblot?
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In eukaryotic cells,post-translational m...View Answer
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