You Constructed a CDNA Library in a Lambda Vector Using

Essay

You constructed a cDNA library in a lambda vector using mRNAs from a pea plant.You want to identify a rubisco (a protein involved in photosynthesis)clone among the 50,000 clones in the library.You have a rubisco cDNA from tobacco to use as a probe.When hybridizing the probe to the plaques in the library,would you use higher,lower,or the same temperature as for hybridizing the tobacco DNA to a tobacco rubisco clone? Why?