Many Enzyme-Catalyzed Reactions That Transfer a Phosphate from One Molecule

Essay

Many enzyme-catalyzed reactions that transfer a phosphate from one molecule to another utilize a histidine residue in the active site of the enzyme. The histidine participates in covalent catalysis by performing a nucleophilic attack on a phosphate, forming an intermediate phosphohistidine (the phosphate covalently attached to the histidine residue). Using ATP as the phosphate donor, show the reaction to form the phosphohistidine intermediate-you do not have to show arrow pushing.