Multiple Choice
Matching
-In ______ chromatography,a protein mixture must be applied to the column at a low pH so that the proteins will have a net positive charge and bind to the column.
A) electrophoresis
B) hydrophobic interaction
C) enzyme-linked immunosorbent assay
D) three-dimensional shape
E) N-terminal amino acid
F) negative charge
G) nucleases
H) chromophore
I) foaming
J) high level expression
K) 2-mercaptoethanol
L) positive charge
M) cation exchange
N) pI
O) chymotrypsin
P) C-terminal amino acid
Q) Sodium dodecyl sulfate
Correct Answer:

Verified
Correct Answer:
Verified
Q20: The vast majority of polypeptides contain between
Q21: Proteins are often constructed from multiple segments
Q22: A fast and common method for determining
Q23: You are purifying a nuclease by affinity
Q24: Enzymes that hydrolyze the internal peptide bonds
Q26: A variety of chromatographic techniques are available
Q27: Which of the following amino acids would
Q28: We are able to purify proteins because
Q29: Matching<br><br>-_ chromatography is a method of fractionating
Q30: Matching<br><br>-In general,proteins are least soluble in water