Essay
You have purified the receptor for a hormone by affinity chromatography.During gel filtration chromatography under native conditions the receptor elutes between pyruvate decarboxylase (250 kDa)and glutamine synthetase (620 kDa).During SDS-PAGE,in the absence of reducing agents,the receptor migrates as a single band of approximately 230 kDa.When SDS-PAGE is carried out in the presence of 2-mercaptoethanol the receptor migrates as two bands of approximately 95 and 135 kDa.Explain this result.
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The receptor is a heterotetramer compose...View Answer
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