DNA Fragments Are Usually Inserted into a Plasmid Vector and Cloned

Multiple Choice

DNA fragments are usually inserted into a plasmid vector and cloned before they are analyzed by chain termination sequencing because

A) DNA fragments need to be pure in order to obtain an unambiguous sequence.
B) a large number of identical fragments is needed in a sequencing reaction in order to generate detectable amounts of products.
C) plasmid vectors have known sequences that can be used for design and annealing of the primer oligonucleotides,which are used to initiate the sequencing reaction.
D) all of the above
E) none of the above