What Observation About Protein Refolding or Renaturation Helped to Solidify

Multiple Choice

What observation about protein refolding or renaturation helped to solidify the connection between primary amino acid sequence and 3-D structure?

A) Spontaneous refolding of proteins into their native state under physiologic conditions.
B) Assisted refolding of proteins into their native state under laboratory conditions.
C) Identification of thermostable proteins than maintain their native state in adverse temperatures.
D) A and B
E) B and C