In the Anfinsen Experiment with the Unfolding of RNaseA,what Order

Multiple Choice

In the Anfinsen experiment with the unfolding of RNaseA,what order could the chemical reagents be removed in order to achieve an inactive protein?

A) Remove denaturant first and reductant second.
B) Simultaneously remove denaturant and reductant.
C) Remove reductant first, denaturant second, and then finally add back reductant.
D) Remove reductant first and denaturant second.