Below Are the Steps of a Western Blot

Multiple Choice

Below are the steps of a Western blot.What is the correct order?
1) The membrane is incubated with the secondary antibody.
2) The membrane is incubated with the primary antibody.
3) The membrane is washed with a blocking solution.
4) Proteins are transferred to a nitrocellulose membrane.
5) Protein extracts are separated by gel electrophoresis.

A) 5,4,3,2,1
B) 4,5,2,3,1
C) 3,4,5,1,2
D) 2,5,4,3,1