Question 1

The expression of cell surface markers is determined in the flow cytometer by which of the following?

Accepted Answer

A)  chemiluminescence 
B)  fluorescence 
C)  optical density 
D)  radioactivity 
E)  scatter B

Question 2

Which is a difference between batch and random access analyzers?

Accepted Answer

A)  Batch analyzers are not automated. 
B)  Random access analyzers only analyze one sample at a time. 
C)  Batch analyzers only do one type of analysis at a time. 
D)  Random access analyzers cannot be used for stat samples. C

Question 3

As measured in flow cytometry,cells that are the smallest and have the least granules would be identified as:

Accepted Answer

A)  lymphocytes 
B)  granulocytes 
C)  monocytes 
D)  erythrocytes A

Question 4

If a test system has many false-positive results,this is a problem involving:

Accepted Answer

A)  accuracy 
B)  precision 
C)  analytic sensitivity 
D)  sampling error

Question 5

A mature cytotoxic T cell expresses which markers?

Accepted Answer

A)  CD3,CD4,and CD8 
B)  CD4,CD3,and CD5 
C)  CD5,CD8,and CD3 
D)  CD8,CD3,and CD2

Question 6

Which of the following is an intrinsic parameter that can be measured by a flow cytometer?

Accepted Answer

A)  surface expression of CD3 
B)  internal complexity 
C)  fluorescence intensity 
D)  nuclear morphology

Question 7

What is meant by "gating" in flow cytometry?

Accepted Answer

A)  the chromatic arrangement of cellular populations 
B)  process by which one cell population adheres to another population 
C)  an electronic window separating subpopulations of cells 
D)  an electronic device measuring all populations of cells

Question 8

In most flow cytometers,labeled cells:

Accepted Answer

A)  scatter the light and absorb fluorescence 
B)  absorb fluorescence and emit electronic impulses 
C)  scatter the light and emit fluorescence 
D)  absorb both fluorescence and light

Question 9

In flow cytometry,if there are two light detectors,what does the amount of side scatter indicate?

Accepted Answer

A)  size of the cell 
B)  granularity of the cell 
C)  surface markers 
D)  shape of the cell

Question 10

A range of values that all produce a positive result is known as:

Accepted Answer

A)  analytic sensitivity 
B)  reportable range 
C)  accuracy 
D)  precision

Exam 12: Flow Cytometry and Laboratory Automation