A PCR Assay Is Performed on a Patient with Presumptive

Multiple Choice

A PCR assay is performed on a patient with presumptive mantle cell lymphoma using primers against the immunoglobulin heavy chain gene and the cyclin D1 (CCND1) gene. Capillary gel electrophoresis was used for product detection, and the patient did not have a detectable PCR product. All controls were acceptable. Which of the following is the correct interpretation of this result?

A) The patient is positive for a t(11;14) .
B) The patient is negative for a t(11;14) .
C) Mantle cell lymphoma is definitively ruled out.
D) The results are a false negative because inappropriate primers were used.