A PCR Assay Using Primers Specific for the Autolysin Gene

Multiple Choice

A PCR assay using primers specific for the autolysin gene (lytA) was performed on a sputum specimen from a 10-year-old girl. The specimen was positive, and all controls were acceptable. Which of the following is the best interpretation of these results?

A) Definitive diagnosis of clinically significant Streptococcus pneumoniae pneumonia is made.
B) Streptococcus pneumoniae is present, but clinical significance is questionable.
C) Mycobacterium tuberculosis is detected, and the girl should be isolated.
D) The patient has Chlamydophila pneumoniae pneumonia.