A Sanger Sequencing Procedure Has Been Performed on a 300-Bp

Multiple Choice

A Sanger sequencing procedure has been performed on a 300-bp piece of DNA. When the fragments were resolved on the gel, there were bands distributed throughout the T, C and A lanes, but in the G lane, there was one thick band at the bottom of the gel. What is the likely cause of these results?

A) No dGTP was added to the G reaction mix.
B) No ddGTP was added to the G reaction mix.
C) There was only one G in the whole 300-bp piece of DNA.
D) The 300-bp piece of DNA contained Gs only.