A PCR Assay Is Performed, and the Results Are Being

Multiple Choice

A PCR assay is performed, and the results are being analyzed. The positive control has a band at the expected size; the negative template control has a band at the same size as the positive control; the contamination control has no band; and the amplification control has a band of expected size. All of the patient samples that were run had a band the same size as the band seen in the positive and negative template control wells. How are these results interpreted?

A) This is an acceptable run, and the patients should all be reported as positive.
B) This run is not acceptable because the amplification control should not have a band.
C) This run is not acceptable because the contamination control should have a band.
D) This run is not acceptable because the negative template control should not have a band.