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After Chromogenic Signal Detection on a Northern Blot, Circular Colorless

Question 23

Multiple Choice

After chromogenic signal detection on a northern blot, circular colorless areas are observed on the membrane, obliterating bands and parts of bands. What is the cause of this?


A) Incomplete electrophoresis of the nucleic acid
B) Loss of alkaline phosphatase activity
C) Incorrect stringency conditions for probe binding
D) Bubbles or incomplete contact between the membrane and the gel

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