Which of the Following Is the Major Disadvantage of RT-PCR

Multiple Choice

Which of the following is the major disadvantage of RT-PCR?

A) DNA is labile and therefore all DNA-based testing require meticulous care to avoid degradation.
B) Large sample size is required.
C) Not sensitive to low number of target sequences.
D) RNA is labile and therefore all RNA-based testing requires meticulous care to avoid degradation by RNases.
E) All of the above