You Discover What Looks to Be a New Organelle That

Multiple Choice

You discover what looks to be a new organelle that is smaller than a mitochondrion, but you need to do further testing to best determine its cellular function.What would be your best plan of action?

A) Use a light microscope under normal culturing conditions, and watch the organelle's movement through the cell.
B) Observe the organelle with freeze-fracture electron microscopy.
C) Isolate the organelle using cell fractionation, and follow with further biochemical analysis.
D) Use a chemical against actin to prevent organelle transport within the cell and observe the resulting cellular function.
E) Use a detergent to break up the lipid membrane of the organelle in the cell, and observe the resulting cell function.