In an Experiment, the F₁ Portion of the ATP Synthase

Multiple Choice

In an experiment, the F₁ portion of the ATP synthase was labeled with a fluorescently labeled microfilament and attached to a glass slide.The rotation of the ATP synthase was observed when ATP was added.When ATP plus oligomycin was added to the slide, the rotation continued even though oligomycin can inhibit ATP hydrolysis in mitochondrial inner membrane vesicles containing ATP synthase (F₁-F₀) .Based on your knowledge of ATP synthase, which hypothesis is supported by these observations?

A) Oligomycin binds to ATP synthase (F₁) and interferes with the rotation.
B) Oligomycin binds to ATP synthase (F₁) on the slide because the binding site is not blocked.
C) Oligomycin binds the ATP synthase (F₁-F₀) only when it is attached to the membrane.
D) Oligomycin binds to the slide.
E) Oligomycin cannot bind to the ATP synthase (F₁-F₀) when it is attached to the membrane.