Under Which Circumstance Would a Researcher Most Likely Use Sanger

Multiple Choice

Under which circumstance would a researcher most likely use Sanger sequencing instead of high-throughput sequencing?

A) If the researcher is undertaking a comparative genomics study and is sequencing prokaryotes
B) If the researcher is undertaking a comparative genomics study and is sequencing eukaryotes
C) If the researcher is doing a metagenomics study
D) If the researcher is well funded and plans to sequence a large number of organisms
E) If the researcher has only modest funds, lacks miniaturization equipment, and plans to sequence only a relatively small amount