The DNA Sequence 5ʹ-AGCTCA-3ʹ Is Not a Palindromic Restriction Site

Multiple Choice

The DNA sequence 5ʹ-AGCTCA-3ʹ is not a palindromic restriction site.Which mutation, if any, at the A at the 3ʹ end could make this into a restriction site?

A) Only a change from A to T
B) Only a change from A to C
C) Only a change from A to G
D) Any single mutation at that position
E) No single mutation at that position