Which Is the Best Representation of a Nucleotide Substitution

Multiple Choice

Which is the best representation of a nucleotide substitution?

A) There is a mutation at a nucleotide from A to G that is quickly lost from the population.
B) There is a mutation at a nucleotide from T to C. Over the course of time, it reaches 100 percent frequency in the species.
C) There is an insertion of 14 nucleotides in a gene. Over the course of time, it reaches 100 percent frequency in the species.
D) Alignment of two sequences indicates differences in sequence length.
E) There is a duplication event of a gene.