Which Scenario Would Tend to Make Obtaining Correct Sequence Alignments

Multiple Choice

Which scenario would tend to make obtaining correct sequence alignments difficult?

A) Comparing closely related species
B) Examining slowly-evolving sequences
C) Working with a segment of DNA in which deletions frequently evolve
D) Knowing which types of substitutions are most likely to evolve
E) Having sequences of a gene from several species at different degrees of evolutionary relatedness from one another