The Polymerase Chain Reaction (PCR) Allows Researchers to Amplify a Gene

Multiple Choice

The polymerase chain reaction (PCR) allows researchers to amplify a gene of interest in a reaction using temperature cycling, salts, and various buffers and reagents.Assess why the DNA polymerase from Thermus aquaticus was instrumental in the development of PCR.

A) Its DNA polymerase is stable and functional at the high temperatures required during PCR cycling.
B) Its DNA polymerase is capable of generating DNA from an RNA precursor.
C) It is the only polymerase that also demonstrates exonuclease activity.
D) Its DNA polymerase is stable at a very low pH.
E) Its DNA polymerase is stable at very high salt concentrations.