How Might a Change of One Amino Acid at a Site

Multiple Choice

How might a change of one amino acid at a site, distant from the active site of an enzyme, alter an enzyme's substrate specificity?

A) by changing the enzyme's stability
B) by changing the enzyme's location in the cell
C) by changing the shape of an enzyme
D) by changing the enzyme's pH optimum
E) An amino acid change away from the active site cannot alter the enzyme's substrate specificity.