In a Single Polymerase Chain Reaction (PCR) Cycle Consisting of 15

Multiple Choice

In a single polymerase chain reaction (PCR) cycle consisting of 15 seconds at 94°C, 30 seconds at 50°C, and 1 minute at 72°C, what is happening in the step run at 50°C?

A) The DNA to be amplified is being denatured.
B) Primers are being denatured.
C) DNA polymerase is extending new DNA from the primers.
D) Primers are annealing to the DNA to be amplified.
E) DNA polymerase is being inactivated.