All of the Following Statements About the Michaelis Constant Are

Multiple Choice

All of the following statements about the Michaelis constant are correct EXCEPT which one?

A) For many enzymes, Km approximates the equilibrium constant for the conversion of ES to E and P.
B) A low value for Km is generally associated with a high affinity of an E for an S.
C) Km is calculated from the intersection, in a Lineweaver-Burk plot, of the experimentally determined line with the horizontal axis.
D) Km may be defined as the substrate concentration which gives half-maximal velocity.
E) At a substrate concentration equal to Km, half of the total number of enzyme molecules may be present as an ES complex.