In the Whole-Genome Shotgun Method, the Genome Is Fragmented and Individual

Multiple Choice

In the whole-genome shotgun method, the genome is fragmented and individual fragments are sequenced. How do we determine the order of the nucleotides in the intact chromosome?

A) The fragmentation is done in a very systematic way such that the physical arrangement of fragments is readily apparent.
B) The sequences of the ends of the fragments overlap with the ends of other fragments.
C) We supplement our information with data from a different technique to determine the final chromosome arrangement.
D) DNA hybridization assays are conducted to determine the physical arrangement of the genes on the chromosome.