EcoRI Makes Staggered Cuts When It Cleaves DNA, Creating Single-Stranded

Multiple Choice

EcoRI makes staggered cuts when it cleaves DNA, creating single-stranded tails called "sticky ends." These ends can form a complementary base pair. In order for this to happen, which of the following conditions is necessary?

A) The presence of specific helicases
B) High enough temperatures
C) Methyl groups at each end
D) Sufficiently low temperature
E) None of the above