What Is the Major Disadvantage of Hierarchical Sequencing as Compared

Multiple Choice

What is the major disadvantage of hierarchical sequencing as compared to shotgun sequencing?

A) Hierarchical sequencing requires more sophisticated computer algorithms.
B) Hierarchical sequencing requires special restriction enzymes, whereas shotgun sequencing does not.
C) Hierarchical sequencing requires more steps than shotgun sequencing, and hence is slower.
D) Both a and b are disadvantages.
E) None of the above is a disadvantage.