The Correct Sequence of Procedures in the Southern Blotting (Hybridization)

Multiple Choice

The correct sequence of procedures in the Southern blotting (hybridization) technique is:
A.hybridization with radioactive probE.
B.agarose gel electrophoresis and visualize bands.
C.transfer (blot) to nitrocellulose filter.
D.digest DNA with restriction nucleases.
E.expose filter to X-ray film, develop and observe.

A) B, A, C, E, D
B) D, C, B, A, E
C) C, D, B, E, A
D) D, B, C, A, E
E) A, B, C, D, E