Which of the Following Items Was One of the Crucial

Multiple Choice

Which of the following items was one of the crucial elements of the Anfinsen experiment with ribonuclease A?

A) hydrophobic interactions were disrupted by the addition of β-mercaptoethanol
B) correct formation of disulfide bonds was achieved even with urea present
C) removal of β-mercaptoethanol resulted in complete denaturation of the protein
D) the presence of 8 cysteine residues means that there are 105 different disulfide bond possibilities
E) none of the above