Which of the Following Is NOT Part of Initiation of Transcription

Multiple Choice

Which of the following is NOT part of initiation of transcription in eukaryotes?

A) Phosphorylation of Ser-5 in the heptad repeats in the C-terminal domain of RNA polymerase II by TF-IIH.
B) Unwinding of the DNA template by TF-IIH
C) Non-specific interactions of general transcription factors at promoters
D) Phosphorylation of Ser-2 in the heptad repeats in the C-terminal domain of RNA polymerase II by P-TEFb.
E) The binding of upstream regulatory proteins