Which of the Following Is NOT Correct When Considering the Initiation

Multiple Choice

Which of the following is NOT correct when considering the initiation of transcription in E. coli?

A) The σ subunit of RNA polymerase makes strong contacts with the -10 region of promoters.
B) Only the -10 region of the promoter is required.
C) DNA melting near the transcription start site is essential for promoter recognition.
D) The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated.
E) The RNA polymerase contacts about 50 bp of DNA.