Evidence Supporting E

Multiple Choice

Evidence supporting E.coli DNA polymerase III having the major role in nucleotide incorporation during replication includes:

A) DNA polymerase III has a high Vmax.
B) There are ten or fewer molecules of enzyme per cell.
C) Replication is blocked at high temperatures in temperature sensitive mutants with a thermolabile form of DNA polymerase III.
D) A point mutation can inactivate DNA pol III and inhibit replication.
E) All of the above.